绍兴文理学院二OO二学年第一学期

  生物科学专业___99__级《细胞生物学》期末考试题（A）

 

题  号

（型）

一

二

三

四

五

六

核分人

 

得  分

 

 

 

 

 

 

总  分

 

 

得  分

 

评卷人

 

 

一、选择填空（每小题2分，共60分，请做在答题卡上）

1．The first man that described the cell structures is  

  1. Robert Hook.
  2. van Leeuwenhoek.
  3. W. Flemming.
  4. J. E. Pukinye.

2．Phase contrast microscope can be used to observe  

A.     Alive cell.

B.     Stained cell.

C.    Not stained cell.

D.    All of the above.

3．If you wanted to separate eukaryote ribosomal subparticles by size, which of the following techniques would you use?  

A.     Gel electrophoresis. 

B.     Radiolabelling.

C.    Polymerase chain reaction.

D.    Density gradient centrifugation.

4．Which of the following description about prion in not true?  

  1. Prion is a sort of protein that causing some severe diseases, such as bovine sponge-form encephalopathies (BSM, mad cow disease).
  2. Prion can replicate itself by change the conformation of the proteins which are similar to prion.
  3. Prion is a transcription regulatory protein.
  4. Prion was discovered by Prusiner in 1982.

5．In general, glycoproteins and glycolipids:  

  1. Are on the outer leaflet of the unit membrane, facing the environment of a cell.
  2. Are on the inner leaflet of the unit membrane, facing the cytosol.
  3. Are equally distributed on the inner and outer surface of the unit membrane.
  4. Occur only in internal membranes.

6．Which of the following is not the function of the bio-membrane?  

  1. Signal transduction.
  2. Transportation.
  3. Transcription.
  4. Compartmentalization.

7．In human intestine glucose are absorbed by the way of  

  1. Na+ powered antiport.
  2. Na+ powered symport.
  3. Channel proteins.
  4. ABC transporter.

8．The Na+/K+ ATPase of eukaryotic cells  

  1. Is a P-type pump.
  2. Is a V- type pump.
  3. Is an F- type pump.
  4. Is an ATP-binding cassette.

9．Following organelles are all surrounded by double bio-membranes except    

  1. Mitochondrion
  2. Chloroplast.
  3. ER.
  4. Nucleus.

10．"Cis," "medial "and "trans " specify different compartments of the...

  1. Golgi body.
  2. Endoplasmic reticulum.
  3. Mitochondrion.
  4. Nucleus.

11．The PH value in lysosome is lower than that in cytoplasm because lysosome membrane exist  

  1. P-type H+ pump.
  2. F-type H+ pump.
  3. V-type H+ pump.
  4. P-type Na+-K+ pump.

12．Which of the following statements about signal sequences is false?    

  1. Signal sequences are removed from protein by signal peptidase after the sorting process has been completed (except nuclear localization sequence (NLS) and signal patch).
  2. Each signal sequence specifies a particular destination in the cell.
  3. NLS direct proteins into cytoplasm from nucleus.
  4. Any protein with a proper signal sequence will be transferred into the peroxisome.

13．Which of the following about peroxisomes is true  

  1. They are formed by binary fission of existing peroxisomes.
  2. They contain their own DNA.
  3. They contain oxidative enzymes.
  4. Both A and C

14．PSI absorb light of wavelength at  

A.     680nm.

B.     700nm

C.    280nm

D.    Both A and B

15．Which of the following is not encoded by the mtDNA?  

  1. Cytochrome c.
  2. Cytochrome b.
  3. F0.
  4. Keratin.

16．Cyclic photophosphorylation is accomplished by  

  1. Photosystem I.
  2. Photosystem II.
  3. Photosystem III.
  4. Both photosystems I and II.

17．G-protein linked receptors are   

  1. Single-pass transmembrane proteins.
  2. Seven-pass transmembrane proteins.
  3. Three-pass transmembrane proteins.
  4. Not a transmembrane protein.

18．Which of the following is NOT a second messenger?    

  1. NO.
  2. cGMP.
  3. cAMP.
  4. IP3.

19．Dyneins are motor proteins that move from   along    

  1. –end to +end; microtubules.
  2. +end to –end; microtubules.
  3. –end to +end; microfilaments.
  4. +end to –end; microfilaments.

20．Microtubules function in cells  

  1. As structural supports, maintaining the shape of the cell.
  2. To form the spindle during mitosis and meiosis.
  3. As a part of the system that causes movement in cilia and flagella.
  4. All of the above.

21．Functions of tight junctions include all the following except    

  1. Separation of extracellular fluid.
  2. Sealing of body cavities.
  3. Prevention of diffusion of membrane proteins and lipids between apical and basolateral regions.
  4. Tight communication and extrange of small molecules between neighboring cells.

22．Which of the following cytoskeleton structures links to desmosomes and hemidesmosomes?    

  1. Microtubules.
  2. Intermediate filaments.
  3. Microfilaments.
  4. all of the above.

23．Cell binding domain in fibronectins contain  

  1. RGD sequences.
  2. KDEL sequences.
  3. Gly-XY sequences.
  4. PEST sequences.

24．The core proteins in nucleosome are  

  1. H2A, H2B, H3, H4.
  2. H1A, H1B, H3, H4.
  3. H1A, H2A, H3, H4.
  4. H1, H2, H3, H4.

25．Eukaryotic chromosomes contain two general domains which relate to the degree of condensation. These two regions are  

  1. Uniform in the genetic information they contain.
  2. Called heterochromatin and euchromatin.
  3. Separated by large stretches of repetitive DNA.
  4. Both A and B

26．What is the main function of cyclin proteins?  

  1. They initiate a signal transduction cascade that leads to changes in the cell’s behavior.
  2. They regulate the cell division by binding and activating CDK enzymes.
  3. They are the main protein components of the kinetochore.
  4. They block cell cycle progression when conditions are not appropriate for growth.

27．MPF consists of 

  1. CDC2 and cyclin D
  2. CDC2 and cyclin E
  3. CDC2 and cyclin B
  4. CDK1 and cyclin C

28．Necrosis  

  1. May be mediated by FAS signal pathway.
  2. Characterized by DNA degraded into about 200bp fragment.
  3. Is a genetically controlled process.
  4. Occurs when cell is injured.

29．Which of the following mutations in a tumor suppressor gene will most likely not result in cancer?  

  1. Point mutant.
  2. Deletion.
  3. Gene rearrangement.
  4. All of the above.

30．Which of the following about apoptosis is not true?  

  1. Is accompanied by random DNA degradation.
  2. Is a sort of "cellular self-destruction" that takes place in normal tissues.
  3. Is characterized by nuclear fragmentation and cellular breakdown into apoptotic vesicles. And does not induce inflammation.
  4. Differs from necrosis because necrosis occurs when cell is injured.

 

得  分

 

评卷人

 

 

二、简答题（20分）

31．用细胞松弛素B处理分裂期的动物细胞将会产生什么现象？为什么？（5分）

关键词：Myosin II, Actin

 

 

 

 

32．什么是Hayflick极限？有什么理论依据？（5分）

关键词：成纤维细胞 端粒 端聚酶

 

 

 

 

33．说明减数分裂的遗传学意义。（10分）

关键词：联会 重组

 

 

 

 

 

 

得  分

 

评卷人

 

 

三、论述题（20分）

34．论述生物膜的结构及其在生命活动中的作用。

关键词：质膜 内膜系统 功能区隔 跨膜运输 信号转导 能量转换 酶

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

35．从细胞周期调控的角度论述肿瘤的发生。

关键词：CDK, CDC, cyclin, CKI p53, p21, 原癌基因 抑癌基因 生长因子 信号通路（signal pathway） 转录因子